ta cloning vector pmd19 t (TaKaRa)
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ta cloning vector pmd19 t
Ta Cloning Vector Pmd19 T, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 8262 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ta+cloning+vector+pmd19+t/T-Vector+pMD+19/10__3390_slash_genes16080912-61-24-28
Average 97 stars, based on 8262 article reviews
Ta Cloning Vector Pmd19 T, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 8262 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ta+cloning+vector+pmd19+t/T-Vector+pMD+19/10__3390_slash_genes16080912-61-24-28
Average 97 stars, based on 8262 article reviews
ta cloning vector pmd19 t - by Bioz Stars,
2026-09
97/100 stars
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Polymerase Chain Reaction:Article Title: Tissue-specific promoters regulate the transcription of cyp19a1 in the brain-pituitary-gonad axis of Japanese eel (Anguilla japonica). Article Snippet: Aromatase is a key enzyme that catalyzes the biosynthesis of estrogens.. Previous study indicated that putative tissue-specific promoters of the one aromatase gene (cyp19a1) may drive the differential regulatory mechanisms of cyp19a1 expression in Anguilla japonica.. In the present study, for elucidating the transcription characteristics and the function of putative tissue-specific promoters of cyp19a1 in the brain-pituitary-gonad (BPG) axis during vitellogenesis, we investigated the transcriptional regulation of cyp19a1 by 17β-estrogen (E2), testosterone (T), or human chorionic gonadotropin (HCG) in A. japonica. Article Title: Comparative analysis of oncogenic genes revealed unique evolutionary features of field Marek's disease virus prevalent in recent years in China Article Snippet: .. Purified PCR products were ligated with a Article Title: Deciphering the structure, function, and mechanism of lysine acetyltransferase cGNAT2 in cyanobacteria. Article Snippet: .. The PCR products were cloned into the Article Title: Involvement of Methoprene-tolerant and Krüppel homolog 1 in juvenile hormone-mediated vitellogenesis of female Liposcelis entomophila (End.) (Psocoptera: Liposcelididae). Article Snippet: The PCR program was 95°C for 3min, 35 cycles of denaturing at 98°C for 10 s, annealing at 56°C–62°C (based on the primers' annealing temperature) for 5 s and extension at 72°C for 45 s, with a final extension at 72°C for 2min using PrimeSTAR Max DNA polymerase (Takara) according to manufacturer's protocol. .. The PCR amplification products were then electrophoresed on a 1% agarose gel, extracted, purified with a gel purification kit (Axygen), and cloned into a Article Title: Involvement of Methoprene‐tolerant and Krüppel homolog 1 in juvenile hormone‐mediated vitellogenesis of female Liposcelis entomophila (End.) (Psocoptera: Liposcelididae) Article Snippet: The PCR program was 95°C for 3 min, 35 cycles of denaturing at 98°C for 10 s, annealing at 56°C–62°C (based on the primers' annealing temperature) for 5 s and extension at 72°C for 45 s, with a final extension at 72°C for 2 min using PrimeSTAR Max DNA polymerase (Takara) according to manufacturer's protocol. .. The PCR amplification products were then electrophoresed on a 1% agarose gel, extracted, purified with a gel purification kit (Axygen), and cloned into a TA Cloning:Article Title: Tissue-specific promoters regulate the transcription of cyp19a1 in the brain-pituitary-gonad axis of Japanese eel (Anguilla japonica). Article Snippet: Aromatase is a key enzyme that catalyzes the biosynthesis of estrogens.. Previous study indicated that putative tissue-specific promoters of the one aromatase gene (cyp19a1) may drive the differential regulatory mechanisms of cyp19a1 expression in Anguilla japonica.. In the present study, for elucidating the transcription characteristics and the function of putative tissue-specific promoters of cyp19a1 in the brain-pituitary-gonad (BPG) axis during vitellogenesis, we investigated the transcriptional regulation of cyp19a1 by 17β-estrogen (E2), testosterone (T), or human chorionic gonadotropin (HCG) in A. japonica. Article Title: Special Regulation of GhANT in Ovules Increases the Size of Cotton Seeds Article Snippet: The reactions were duplicated 3 times, and the data were analyzed using the Bio-Rad CFX Manager 2.0 software provided by the manufacturer. .. The GhANT gene was amplified from upland cotton cDNA using a forward primer (5′- ATGAAGTCCATGAGCAATGA-3′) and a reverse primer (5′-ACTGCCTGGACAGATGCTTAG3′) and cloned into the Article Title: Comparative analysis of oncogenic genes revealed unique evolutionary features of field Marek's disease virus prevalent in recent years in China Article Snippet: .. Purified PCR products were ligated with a Article Title: Deciphering the structure, function, and mechanism of lysine acetyltransferase cGNAT2 in cyanobacteria. Article Snippet: .. The PCR products were cloned into the Article Title: Involvement of Methoprene-tolerant and Krüppel homolog 1 in juvenile hormone-mediated vitellogenesis of female Liposcelis entomophila (End.) (Psocoptera: Liposcelididae). Article Snippet: The PCR program was 95°C for 3min, 35 cycles of denaturing at 98°C for 10 s, annealing at 56°C–62°C (based on the primers' annealing temperature) for 5 s and extension at 72°C for 45 s, with a final extension at 72°C for 2min using PrimeSTAR Max DNA polymerase (Takara) according to manufacturer's protocol. .. The PCR amplification products were then electrophoresed on a 1% agarose gel, extracted, purified with a gel purification kit (Axygen), and cloned into a Article Title: Special Regulation of GhANT in Ovules Increases the Size of Cotton Seeds Article Snippet: The reactions were duplicated 3 times, and the data were analyzed using the Bio-Rad CFX Manager 2.0 software provided by the manufacturer. .. The GhANT gene was amplified from upland cotton cDNA using a forward primer (5′-ATGAAGTCCATGAGCAATGA-3′) and a reverse primer (5′-ACTGCCTGGACAGATGCTTAG-3′) and cloned into the Article Title: Gibberellin Overproduction Promotes Sucrose Synthase Expression and Secondary Cell Wall Deposition in Cotton Fibers Article Snippet: .. The BAN promoter was amplified from Arabidopsis with a forward primer ( 5′-TCTAGATAACAGAACCTTAC TGTAACACTATT-3′ ) and a reverse primer (5′-ACTAGTGATTGTACTTTTGAAATTACAGAG AT-3′) and cloned into TA cloning Article Title: Involvement of Methoprene‐tolerant and Krüppel homolog 1 in juvenile hormone‐mediated vitellogenesis of female Liposcelis entomophila (End.) (Psocoptera: Liposcelididae) Article Snippet: The PCR program was 95°C for 3 min, 35 cycles of denaturing at 98°C for 10 s, annealing at 56°C–62°C (based on the primers' annealing temperature) for 5 s and extension at 72°C for 45 s, with a final extension at 72°C for 2 min using PrimeSTAR Max DNA polymerase (Takara) according to manufacturer's protocol. .. The PCR amplification products were then electrophoresed on a 1% agarose gel, extracted, purified with a gel purification kit (Axygen), and cloned into a Amplification:Article Title: Special Regulation of GhANT in Ovules Increases the Size of Cotton Seeds Article Snippet: The reactions were duplicated 3 times, and the data were analyzed using the Bio-Rad CFX Manager 2.0 software provided by the manufacturer. .. The GhANT gene was amplified from upland cotton cDNA using a forward primer (5′- ATGAAGTCCATGAGCAATGA-3′) and a reverse primer (5′-ACTGCCTGGACAGATGCTTAG3′) and cloned into the Article Title: Involvement of Methoprene-tolerant and Krüppel homolog 1 in juvenile hormone-mediated vitellogenesis of female Liposcelis entomophila (End.) (Psocoptera: Liposcelididae). Article Snippet: The PCR program was 95°C for 3min, 35 cycles of denaturing at 98°C for 10 s, annealing at 56°C–62°C (based on the primers' annealing temperature) for 5 s and extension at 72°C for 45 s, with a final extension at 72°C for 2min using PrimeSTAR Max DNA polymerase (Takara) according to manufacturer's protocol. .. The PCR amplification products were then electrophoresed on a 1% agarose gel, extracted, purified with a gel purification kit (Axygen), and cloned into a Article Title: Special Regulation of GhANT in Ovules Increases the Size of Cotton Seeds Article Snippet: The reactions were duplicated 3 times, and the data were analyzed using the Bio-Rad CFX Manager 2.0 software provided by the manufacturer. .. The GhANT gene was amplified from upland cotton cDNA using a forward primer (5′-ATGAAGTCCATGAGCAATGA-3′) and a reverse primer (5′-ACTGCCTGGACAGATGCTTAG-3′) and cloned into the Article Title: Gibberellin Overproduction Promotes Sucrose Synthase Expression and Secondary Cell Wall Deposition in Cotton Fibers Article Snippet: .. The BAN promoter was amplified from Arabidopsis with a forward primer ( 5′-TCTAGATAACAGAACCTTAC TGTAACACTATT-3′ ) and a reverse primer (5′-ACTAGTGATTGTACTTTTGAAATTACAGAG AT-3′) and cloned into TA cloning Article Title: Involvement of Methoprene‐tolerant and Krüppel homolog 1 in juvenile hormone‐mediated vitellogenesis of female Liposcelis entomophila (End.) (Psocoptera: Liposcelididae) Article Snippet: The PCR program was 95°C for 3 min, 35 cycles of denaturing at 98°C for 10 s, annealing at 56°C–62°C (based on the primers' annealing temperature) for 5 s and extension at 72°C for 45 s, with a final extension at 72°C for 2 min using PrimeSTAR Max DNA polymerase (Takara) according to manufacturer's protocol. .. The PCR amplification products were then electrophoresed on a 1% agarose gel, extracted, purified with a gel purification kit (Axygen), and cloned into a Clone Assay:Article Title: Special Regulation of GhANT in Ovules Increases the Size of Cotton Seeds Article Snippet: The reactions were duplicated 3 times, and the data were analyzed using the Bio-Rad CFX Manager 2.0 software provided by the manufacturer. .. The GhANT gene was amplified from upland cotton cDNA using a forward primer (5′- ATGAAGTCCATGAGCAATGA-3′) and a reverse primer (5′-ACTGCCTGGACAGATGCTTAG3′) and cloned into the Article Title: Deciphering the structure, function, and mechanism of lysine acetyltransferase cGNAT2 in cyanobacteria. Article Snippet: .. The PCR products were cloned into the Article Title: Involvement of Methoprene-tolerant and Krüppel homolog 1 in juvenile hormone-mediated vitellogenesis of female Liposcelis entomophila (End.) (Psocoptera: Liposcelididae). Article Snippet: The PCR program was 95°C for 3min, 35 cycles of denaturing at 98°C for 10 s, annealing at 56°C–62°C (based on the primers' annealing temperature) for 5 s and extension at 72°C for 45 s, with a final extension at 72°C for 2min using PrimeSTAR Max DNA polymerase (Takara) according to manufacturer's protocol. .. The PCR amplification products were then electrophoresed on a 1% agarose gel, extracted, purified with a gel purification kit (Axygen), and cloned into a Article Title: Special Regulation of GhANT in Ovules Increases the Size of Cotton Seeds Article Snippet: The reactions were duplicated 3 times, and the data were analyzed using the Bio-Rad CFX Manager 2.0 software provided by the manufacturer. .. The GhANT gene was amplified from upland cotton cDNA using a forward primer (5′-ATGAAGTCCATGAGCAATGA-3′) and a reverse primer (5′-ACTGCCTGGACAGATGCTTAG-3′) and cloned into the Article Title: Gibberellin Overproduction Promotes Sucrose Synthase Expression and Secondary Cell Wall Deposition in Cotton Fibers Article Snippet: .. The BAN promoter was amplified from Arabidopsis with a forward primer ( 5′-TCTAGATAACAGAACCTTAC TGTAACACTATT-3′ ) and a reverse primer (5′-ACTAGTGATTGTACTTTTGAAATTACAGAG AT-3′) and cloned into TA cloning Article Title: Involvement of Methoprene‐tolerant and Krüppel homolog 1 in juvenile hormone‐mediated vitellogenesis of female Liposcelis entomophila (End.) (Psocoptera: Liposcelididae) Article Snippet: The PCR program was 95°C for 3 min, 35 cycles of denaturing at 98°C for 10 s, annealing at 56°C–62°C (based on the primers' annealing temperature) for 5 s and extension at 72°C for 45 s, with a final extension at 72°C for 2 min using PrimeSTAR Max DNA polymerase (Takara) according to manufacturer's protocol. .. The PCR amplification products were then electrophoresed on a 1% agarose gel, extracted, purified with a gel purification kit (Axygen), and cloned into a Plasmid Preparation:Article Title: Special Regulation of GhANT in Ovules Increases the Size of Cotton Seeds Article Snippet: The reactions were duplicated 3 times, and the data were analyzed using the Bio-Rad CFX Manager 2.0 software provided by the manufacturer. .. The GhANT gene was amplified from upland cotton cDNA using a forward primer (5′- ATGAAGTCCATGAGCAATGA-3′) and a reverse primer (5′-ACTGCCTGGACAGATGCTTAG3′) and cloned into the Article Title: Comparative analysis of oncogenic genes revealed unique evolutionary features of field Marek's disease virus prevalent in recent years in China Article Snippet: .. Purified PCR products were ligated with a Article Title: Deciphering the structure, function, and mechanism of lysine acetyltransferase cGNAT2 in cyanobacteria. Article Snippet: .. The PCR products were cloned into the Article Title: Involvement of Methoprene-tolerant and Krüppel homolog 1 in juvenile hormone-mediated vitellogenesis of female Liposcelis entomophila (End.) (Psocoptera: Liposcelididae). Article Snippet: The PCR program was 95°C for 3min, 35 cycles of denaturing at 98°C for 10 s, annealing at 56°C–62°C (based on the primers' annealing temperature) for 5 s and extension at 72°C for 45 s, with a final extension at 72°C for 2min using PrimeSTAR Max DNA polymerase (Takara) according to manufacturer's protocol. .. The PCR amplification products were then electrophoresed on a 1% agarose gel, extracted, purified with a gel purification kit (Axygen), and cloned into a Article Title: Special Regulation of GhANT in Ovules Increases the Size of Cotton Seeds Article Snippet: The reactions were duplicated 3 times, and the data were analyzed using the Bio-Rad CFX Manager 2.0 software provided by the manufacturer. .. The GhANT gene was amplified from upland cotton cDNA using a forward primer (5′-ATGAAGTCCATGAGCAATGA-3′) and a reverse primer (5′-ACTGCCTGGACAGATGCTTAG-3′) and cloned into the Article Title: Gibberellin Overproduction Promotes Sucrose Synthase Expression and Secondary Cell Wall Deposition in Cotton Fibers Article Snippet: .. The BAN promoter was amplified from Arabidopsis with a forward primer ( 5′-TCTAGATAACAGAACCTTAC TGTAACACTATT-3′ ) and a reverse primer (5′-ACTAGTGATTGTACTTTTGAAATTACAGAG AT-3′) and cloned into TA cloning Article Title: Involvement of Methoprene‐tolerant and Krüppel homolog 1 in juvenile hormone‐mediated vitellogenesis of female Liposcelis entomophila (End.) (Psocoptera: Liposcelididae) Article Snippet: The PCR program was 95°C for 3 min, 35 cycles of denaturing at 98°C for 10 s, annealing at 56°C–62°C (based on the primers' annealing temperature) for 5 s and extension at 72°C for 45 s, with a final extension at 72°C for 2 min using PrimeSTAR Max DNA polymerase (Takara) according to manufacturer's protocol. .. The PCR amplification products were then electrophoresed on a 1% agarose gel, extracted, purified with a gel purification kit (Axygen), and cloned into a Purification:Article Title: Comparative analysis of oncogenic genes revealed unique evolutionary features of field Marek's disease virus prevalent in recent years in China Article Snippet: .. Purified PCR products were ligated with a Article Title: Involvement of Methoprene-tolerant and Krüppel homolog 1 in juvenile hormone-mediated vitellogenesis of female Liposcelis entomophila (End.) (Psocoptera: Liposcelididae). Article Snippet: The PCR program was 95°C for 3min, 35 cycles of denaturing at 98°C for 10 s, annealing at 56°C–62°C (based on the primers' annealing temperature) for 5 s and extension at 72°C for 45 s, with a final extension at 72°C for 2min using PrimeSTAR Max DNA polymerase (Takara) according to manufacturer's protocol. .. The PCR amplification products were then electrophoresed on a 1% agarose gel, extracted, purified with a gel purification kit (Axygen), and cloned into a Article Title: Involvement of Methoprene‐tolerant and Krüppel homolog 1 in juvenile hormone‐mediated vitellogenesis of female Liposcelis entomophila (End.) (Psocoptera: Liposcelididae) Article Snippet: The PCR program was 95°C for 3 min, 35 cycles of denaturing at 98°C for 10 s, annealing at 56°C–62°C (based on the primers' annealing temperature) for 5 s and extension at 72°C for 45 s, with a final extension at 72°C for 2 min using PrimeSTAR Max DNA polymerase (Takara) according to manufacturer's protocol. .. The PCR amplification products were then electrophoresed on a 1% agarose gel, extracted, purified with a gel purification kit (Axygen), and cloned into a Derivative Assay:Article Title: Deciphering the structure, function, and mechanism of lysine acetyltransferase cGNAT2 in cyanobacteria. Article Snippet: .. The PCR products were cloned into the Agarose Gel Electrophoresis:Article Title: Involvement of Methoprene-tolerant and Krüppel homolog 1 in juvenile hormone-mediated vitellogenesis of female Liposcelis entomophila (End.) (Psocoptera: Liposcelididae). Article Snippet: The PCR program was 95°C for 3min, 35 cycles of denaturing at 98°C for 10 s, annealing at 56°C–62°C (based on the primers' annealing temperature) for 5 s and extension at 72°C for 45 s, with a final extension at 72°C for 2min using PrimeSTAR Max DNA polymerase (Takara) according to manufacturer's protocol. .. The PCR amplification products were then electrophoresed on a 1% agarose gel, extracted, purified with a gel purification kit (Axygen), and cloned into a Article Title: Involvement of Methoprene‐tolerant and Krüppel homolog 1 in juvenile hormone‐mediated vitellogenesis of female Liposcelis entomophila (End.) (Psocoptera: Liposcelididae) Article Snippet: The PCR program was 95°C for 3 min, 35 cycles of denaturing at 98°C for 10 s, annealing at 56°C–62°C (based on the primers' annealing temperature) for 5 s and extension at 72°C for 45 s, with a final extension at 72°C for 2 min using PrimeSTAR Max DNA polymerase (Takara) according to manufacturer's protocol. .. The PCR amplification products were then electrophoresed on a 1% agarose gel, extracted, purified with a gel purification kit (Axygen), and cloned into a Gel Purification:Article Title: Involvement of Methoprene-tolerant and Krüppel homolog 1 in juvenile hormone-mediated vitellogenesis of female Liposcelis entomophila (End.) (Psocoptera: Liposcelididae). Article Snippet: The PCR program was 95°C for 3min, 35 cycles of denaturing at 98°C for 10 s, annealing at 56°C–62°C (based on the primers' annealing temperature) for 5 s and extension at 72°C for 45 s, with a final extension at 72°C for 2min using PrimeSTAR Max DNA polymerase (Takara) according to manufacturer's protocol. .. The PCR amplification products were then electrophoresed on a 1% agarose gel, extracted, purified with a gel purification kit (Axygen), and cloned into a Article Title: Involvement of Methoprene‐tolerant and Krüppel homolog 1 in juvenile hormone‐mediated vitellogenesis of female Liposcelis entomophila (End.) (Psocoptera: Liposcelididae) Article Snippet: The PCR program was 95°C for 3 min, 35 cycles of denaturing at 98°C for 10 s, annealing at 56°C–62°C (based on the primers' annealing temperature) for 5 s and extension at 72°C for 45 s, with a final extension at 72°C for 2 min using PrimeSTAR Max DNA polymerase (Takara) according to manufacturer's protocol. .. The PCR amplification products were then electrophoresed on a 1% agarose gel, extracted, purified with a gel purification kit (Axygen), and cloned into a Transformation Assay:Article Title: Involvement of Methoprene-tolerant and Krüppel homolog 1 in juvenile hormone-mediated vitellogenesis of female Liposcelis entomophila (End.) (Psocoptera: Liposcelididae). Article Snippet: The PCR program was 95°C for 3min, 35 cycles of denaturing at 98°C for 10 s, annealing at 56°C–62°C (based on the primers' annealing temperature) for 5 s and extension at 72°C for 45 s, with a final extension at 72°C for 2min using PrimeSTAR Max DNA polymerase (Takara) according to manufacturer's protocol. .. The PCR amplification products were then electrophoresed on a 1% agarose gel, extracted, purified with a gel purification kit (Axygen), and cloned into a Article Title: Involvement of Methoprene‐tolerant and Krüppel homolog 1 in juvenile hormone‐mediated vitellogenesis of female Liposcelis entomophila (End.) (Psocoptera: Liposcelididae) Article Snippet: The PCR program was 95°C for 3 min, 35 cycles of denaturing at 98°C for 10 s, annealing at 56°C–62°C (based on the primers' annealing temperature) for 5 s and extension at 72°C for 45 s, with a final extension at 72°C for 2 min using PrimeSTAR Max DNA polymerase (Takara) according to manufacturer's protocol. .. The PCR amplification products were then electrophoresed on a 1% agarose gel, extracted, purified with a gel purification kit (Axygen), and cloned into a |
